Chlamydia trachomatis

One-Sentence Definition

Chlamydia trachomatis is an obligate intracellular Gram-negative bacterium with a biphasic developmental cycle that causes STI syndromes, neonatal infection, and trachoma — diagnosed primarily by nucleic acid amplification, not routine culture.

Taxonomy & Morphology

  • Family Chlamydiaceae; related respiratory pathogens: C. pneumoniae, C. psittaci
  • No peptidoglycan-rich wall suitable for Gram stain in practice — “atypical”
  • Elementary body (EB) — infectious, extracellular; reticulate body (RB) — replicating, intracellular
  • Serovars: A–C (trachoma), D–K (urogenital/neonatal), L1–L3 (LGV)

Virulence Highlights

  • Type III secretion and inclusion formation inside epithelial cells
  • Immune evasion via intracellular niche; chronic inflammation drives scarring (fallopian tube, conjunctiva)
  • Antigenic variation of major outer membrane protein (MOMP) among serovars

Clinical Syndromes

  • Urethritis, cervicitis, PID, epididymitis; often asymptomatic
  • Neonatal conjunctivitis / pneumonia
  • Trachoma (leading infectious cause of blindness historically)
  • LGV: invasive inguinal/anorectal disease (MSM networks important)

Diagnosis

  • NAAT / PCR on urine or swab — first-line (Isothermal Nucleic Acid Amplification / syndromic STI panels often include it)
  • Culture rarely used clinically; serology limited (except some LGV contexts)
  • Test-of-cure selectively (pregnancy, persistent symptoms)

Treatment Notes (conceptual)

  • Doxycycline preferred for many urogenital infections; azithromycin alternatives in selected settings
  • Treat partners; screen high-risk populations
  • LGV needs longer courses — distinguish from non-LGV serovars when invasive

AMR

  • Clinical failure more often reinfection/adherence than high-level chromosomal AMR
  • Rising macrolide/tetracycline concern in some regions — surveillance evolving
  • Not an ESKAPE organism; stewardship still matters for empiric STI regimens

Genomics & Computational Notes

  • Small genome (~1 Mb); metabolic dependence on host
  • Serovar/genotype from ompA or WGS for LGV outbreak work
  • Links: Metagenomics less used than targeted NAAT for routine care

Active Recall

  1. EB vs RB — which is infectious extracellularly?
  2. Why is Gram stain useless for diagnosing chlamydial urethritis?
  3. Which serovar group causes LGV?