Episode 3: Where it comes from. Narration (Short, 58 s)
Voice: Azure neural en-US-AndrewMultilingualNeural (as in Two Fates), rate -6 %, pitch -2 Hz, via scripts/make_narration.py -> narration/01..12.wav. Timecodes are film seconds (frame/30).
| # | Time | Picture | Line |
|---|---|---|---|
| 01 | 0:00.5 | The episode-1 enzyme in the periplasm | Where did this enzyme come from? |
| 02 | 0:05.5 | Camera drops into the cytoplasm; the chromosome | Not from the chromosome. |
| 03 | 0:09.5 | The plasmid ring; the gene arc lights | From this. A plasmid: one extra ring of DNA, carrying one gene. |
| 04 | 0:15.6 | A new enzyme forms beside the ring | The gene is read. The protein is made. |
| 05 | 0:19.0 | It crosses the inner membrane and settles in the periplasm | Its signal sequence sends it across the inner membrane, into the periplasm. That is where it waits. |
| 06 | 0:27.0 | Wide: a second cell appears | Next door, a cell with no enzyme. |
| 07 | 0:30.5 | The pilus bridges the cells; a strand runs across; ring B completes | A pilus pulls it close. One strand of the ring crosses, and the neighbour completes it. |
| 08 | 0:38.5 | Gene lights in cell B | Now it has the gene too. |
| 09 | 0:42.5 | Enzyme forms in B and reaches the periplasm | Minutes later, it makes the enzyme. |
| 10 | 0:47.5 | Ceftazidime enters B’s porin | Same drug. Same door. |
| 11 | 0:51.5 | Ring opens on the enzyme | Ring broken. The cell carries on. |
| 12 | 0:55.0 | Wide: both cells, both plasmids | One gene, passed from cell to cell. That is how resistance spreads. |
About 105 words. Simplifications stated in the description: transcription and translation are not shown (the protein simply forms), the plasmid and chromosome are stylized DNA, the pilus is the real F-pilus filament (PDB 5LER) but the transfer channel is not shown.