Episode 2b upload sheet

File: renders/two_fates_v4.mp4 (1920x1080, 102 s, narration + music + SFX). Not a Short.

Title Two Fates: One Enzyme Decides Whether an Antibiotic Works

Description The same antibiotic enters two bacteria through the same door. One cell carries a beta-lactamase, the other does not. Watched side by side, frame for frame, they share the first fifteen seconds and then part: in the first cell the enzyme opens the drug’s beta-lactam ring and releases it, harmless; in the second the drug opens its ring on the cell-wall enzyme PBP1b and stays bound, the wall stops growing, opens, and the inner membrane pushes through.

Everything shown is a real structure. OmpF porin and E. coli LPS come from a molecular dynamics simulation (CHARMM-GUI, GROMACS). TEM-1 beta-lactamase from a 10 ns MD run; the bound and hydrolyzed cephalosporin poses from PDB 5UJ3 placed on the active site. PBP1b from PDB 3VMA; the acyl-enzyme pose from PDB 2C5W placed on the conserved SxxK / SxN / KTG motifs. AcrB from MemProtMD (4DX5). The peptidoglycan mesh and the lysis are stylized. Rendered in Blender with Molecular Nodes.

Narration: synthetic voice (Azure neural). Music: “Rain Over Kyoto Station” by The Mini Vandals (YouTube Audio Library).

Episode 1 (the envelope): https://youtube.com/shorts/kJKye8xqjRg Episode 2 (hitting the wall): https://youtube.com/shorts/ufXzk6dhlX8

0:00 A real bacterial envelope 0:30 Two fates 0:33 Same entry, frame for frame 0:48 Here they part 1:04 The same chemistry, twice 1:14 One wall keeps growing, one fails 1:32 Next episode

#antibiotics #antibioticresistance #betalactamase #microbiology #molecularbiology #penicillin #cephalosporin #blender #molecularnodes

Playlist: Inside a Gram-Negative Bacterium: How Antibiotics Work (and Fail) Audience: not made for kids Thumbnail: thumbs/thumb_A_twocell.jpg (recommended; B macro in the same folder) Visibility: Public Tags: antibiotics, beta-lactamase, antibiotic resistance, penicillin binding protein, molecular dynamics, blender, molecular nodes, microbiology animation